The solvents include petroleum ether (60–90°C), acetone, tetrahydrofuran, ethyl acetate, methanol, dichloromethane (DCM) and pyridine. The method showed the possibility to detect the tested solvents with a linear determination correlation coefficient ( r ) greater than 0.9995 except for petroleum ether …
Nov 12, 2012· Petroleum ether (boiling point 30 °C (86 °F) —60 °C (140 °F)): Petroleum ether is a light solvent that is much more selective than any of the alcohols. Extracting with petroleum ether produces an oil that is twice as potent by weight as oil extracted with alcohol.
3.1.2. Effect of the Ratio of Mixed Solvent of Petroleum Ether and Ethanol The effect of mixed solvent of petroleum ether and ethanol for acid valueon the rate was studied in Table 2. When the ratio of petroleum ether and ethanol was increased from :1 to1 4:1, the crude ELO acid value gradual-ly …
In merane stabilizing assay, crude methanol extracts of rhizomes and fertile foliage fronds and their petroleum ether fractions were found to be very effective for stabilizing erythrocyte merane in hypotonic solution. In case of throolytic study, crude methanol extract of rhizomes and its aqueous fraction exhibited noticeable clot lysis.
Firstly, the total FOSs were precolumn derivatized and then separated by high-speed counter-current chromatography (HSCCC) with two-phase solvent system petroleum ether–n-butanol–methanol–water (3:2:1:4, v/v). Secondly, the obtained compounds were deacetylated and the fructo-oligosaccharides (FOSs) with high purity were obtained. Their
First, 10ml of sulfuric acid, 50ml petroleum ether and 3ml ethyl alcohol added to 250ml sample in separating funnel. The mixture was shaken well to ensure that all the mixture mix well and the oil and grease will be able to separate from the water. When the mixtures mix well, it allowed standing till 2 layers are formed.
methanol. When the ninhydrin is completely dissolved, 960 cc of petroleum ether F (B.P.-30-60*C) is added and stirred for several min-utes. The mixture is poured into a separatory funnel and allowed to stand for five to ten minutes. Two layers are formed; a small …
Sample preparation: To 1 g of the powder, add 50 mL of petroleum ether (30-60°C) , heat and reflux for 1h, filter and discard the petroleum ether solution.Wash the residue with 25 mL petroleum ether, discard the petroleum ether solution. Evaporate the residue to dryness, add 30 mL of methanol, heat and reflux for 1h, filter and use the filtrate as the test solution
How about dimethyl ether, which is a constitutional isomer of ethanol but with an ether rather than an alcohol functional group? We find that diethyl ether is much less soluble in water. Is it capable of forming hydrogen bonds with water? Yes, in fact, it is –the ether oxygen can act as a hydrogen-bond acceptor.
The petroleum ether extract was separated by the solvent system of n-heptane-methanol-acetonitrile (1.5:0.5:0.5, v/v) and n-heptane-methanol (1.5:1, v/v) in gradient elution, yielding a phloroglucinol compound, hyperforin with HPLC purity over 98%.
The ASTM D86 and D1160 standards describe a simple distillation method for measuring the boiling point distribution of crude oil and petroleum products. Using ASTM, D86 boiling points are measured at 10, 30, 50, 70, and 90 vol% distilled. The points are also frequently reported at 0%, 5%, and 95% distilled.
0.5 N methanolic sodium hydroxide (NaOH) (10 g NaOH in 500 mL methanol): mix the solution for 15 minutes with a stir bar. Don’t heat the mixture because methanol is highly flammable. 14% Boron Trifluoride (BF3) in methanol (commercially available from Sigma) HPLC grade petroleum ether (3.3 mg BHT in 100 mL petroleum ether)
China Petroleum Ether(60-90), Petroleum Ether(60-90) from China Supplier
Sep 13, 2012· IFs decreased from 23.56 to 17.37, 6.35 at the dose of 500 μg/ml for petroleum ether and chloroform extracts respectively, to 8.46 and 12.86 at the dose of 30 μg/ml for ethyl acetate and TOF extracts respectively and to 7.50 at the dose of 200 μg/ml for the methanol extract.
Dec 13, 2019· The synthesized methanol can then be transformed into high-value C 2+ compounds, including DME, lower hydrocarbons, and gasoline via the hydrocarbon-pool mechanism in which an organic center is
2 HPLC methods for water- and fat-soluble vitamin analysis Traditional HPLC method Reversed-phase HPLC is a well-suited technique for vitamin analysis.1 In typical regulated HPLC methods2,3 and commonly reported HPLC methods,4,5 water-soluble vitamins …
1. Take about 100 ml acetone extract of plastidial pigments in a separating funnel (500 ml capacity) and add petroleum ether (about 80-100 ml). 2. Add a few ml of dist. water in the mixture and then shake thoroughly and wait till the acetone water layer settles down.
for 30 min at the boiling point of methanol in the presence of 0.5 g ascorbic acid. After cooling the ﬂask, 15 ml of salted water were added and extracted twice with 40 ml of petroleum ether in a separatory funnel. Ether fractions were collected, washed twice with distilled water, and …
4. Mix the extract with methanol and boil , collect the dewaxed methanol extract , evaporate to dryness, collect the residue. 5. Dissolve the residue in petroleum ether , concentrate and allow to stand (Yellow oil gets separated) 6. The residue is subjected to column chromatography on Alumina and elute with n-hexane: ethyl acetate (99:1) 7.
CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): In the present study Momordica dioica Roxb willd (Cucurbitaceae) fruit pulp was successively extracted with petroleum ether, ethyl acetate and methanol. All extracts were vacuum dried to yield the respective extracts. All extracts were evaluated by non-narcotic models like acetic acid induced writhing syndrome test
Discard the petroleum ether extract, expel solvent from the residue and add a quantity of methanol, heat under reflux for 4 hours, and evaporate to dryness. Dissolve the residue in 15 mL of water saturated with n-butanol, transfer to a separator funnel, and extract by shaking with three 20 mL quantities of n-butanol saturated with water.
The purity of all standards was at least 98%. Thirteen batches of ZTP were purchased from China Resources Sanjiu Medical & Pharmaceutical Co. Ltd. (Shenzhen, China). Methanol, acetic acid, petroleum ether, ethyl ether, and ethyl acetate were purchased from Baishi Chemical Industry Co. Ltd. (Tianjin, China) and of analytical grade.
The invention discloses a method for extracting beta-carotene and zeaxanthin from Chinese wolfberry skin. The method comprises the following steps: (1) extraction: drying and crashing Chinese wolfberry skin, adding mixed solvent, adding antioxidant, stirring, extracting and performing vacuum concentration on extracting solution under reduced pressure to obtain extractum; (2) saponifiion
The extraction and re-extraction mixtures were methanol and petroleum ether in a 2:1 ratio, and petroleum ether and diethyl ether (1:1), respectively. The methanol re-moves water from the plant material and the petroleum ether picks up the pigments before they undergo second-ary reactions . The diethyl ether increases the pig-
The acid will not evaporate and will remain in the Pyrex pot. Allow to cool. Take the cooled solution, pour with an equal volume of water and 1/2 volume of petroleum ether into the ether-extraction apparatus (separation funnel). Allow to settle, and drain the ether extract layer. This leaves an ether-extract-acid mix from which the acid must be
17. In order to separate these: Pour 10ml of petroleum ether on the solid mixture. It will dissolve the biphenyl but not the triphenylmethanol. Decant the liquid & rinse the solids with another 2ml of petroleum ether. 18. Add the liquid to the 10 ml portion. Obtain the pure biphenyl (as a side product) by evaporating the petroleum ether over a